Polarized Light Microscopy Polarized Light Microscopy Polarized light is a contrast-enhancing technique that improves the quality of the image obtained with birefringent materials when compared to other techniques such as darkfield and brightfield illumination, differential interference contrast, phase contrast, Hoffman modulation contrast, and fluorescence.
Familiarize yourself with the parts of the microscope. The total magnification using the lenses can be determined by multiplying the objective lens with the ocular lens. What is the total magnification of an item viewed with the: Examine the diaphragm underneath the stage.
Look into the eyepiece, twist it left and right. Notice the line inside that moves as you twist. Some microscopes do not have this, see if you can find one that does in the room.
What do you think this is for? Place the slide of the "letter e" on the stage so that the letter is over the hole and is right side up. Use the scanning objective to view the letter and use the coarse knob to focus. Repeat on the low power objective. Finally, switch to high power.
Remember at this point, you should only use the FINE adjustment knob. Draw the "e" as it appears at each magnification. Drawings should be drawn to scale and you should note the orientation of the e in the viewing field is it upside down or right side up? On scanning you should see the e clearly and it will be taking up about half of the viewing field.
On low poer the e will be taking up almost the entire viewing field, on high you will only see a tiny section of the e which will be a close-up of the ink used to print the letter. Have your partner push the slide to the left while you view it through the lens.
Which direction does th E appear to move? Random Specimens Choose 2 specimens from the box of "common things". You may practice focusing with the high power, but you do not need to sketch it. Label your specimens from the name written on the slide. Generally, just look to see if the drawings are labeled and are a reasonable representation of the item.
Students like looking at things in the lab, allow them time to explore and provide many specimens. View the slide under scanning and then low power.Forensic Firearms Identification is a discipline of Forensic Science that has as a primary concern to identify fired ammunition components as having been fired from a specific firearm.
Introduction to the Microscope PowerPoint Presentation, PPT - DocSlides- Care. Parts. Focusing. Types of Microscopes. Light Microscope -. the models found in most schools, use compound lenses to magnify objects.
The lenses bend or refract light to make the object beneath them appear closer.. ID: In this free online science interactive, students learn the procedures for operating a compound optical light microscope as they would use in a science lab.
©University of Delaware. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs heartoftexashop.comve Commons Attribution-NonCommercial-NoDerivs License. AmScope MC / MC-I 40XX All-Metal Optical Glass Lenses Cordless LED Student Biological Compound Microscope This is a perfect package of a high power microscope .
Anatomy of the Microscope - Introduction Total internal reflection fluorescence microscopy (TIRFM) is an elegant optical technique utilized to observe single molecule fluorescence at surfaces and interfaces.